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Anti-phospho-EGFR (Tyr1086) Antibody, clone 8B8
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REFERENCES
Tumor-initiating cells of HER2-positive carcinoma cell lines express the highest oncoprotein levels and are sensitive to trastuzumab.
Magnifico, Alessandra, et al. (2009) Clin. Cancer Res., 15: 2010-21 (2009)
Identification of a novel autophosphorylation site (P4) on the epidermal growth factor receptor
Hsuan, J J, et al (1989) Biochem J, 262:659-63 (1989)
Modulation of survival signaling pathways and persistence of the genotoxic stress as a basis for the synergistic interaction between the atypical retinoid ST1926 and the epidermal growth factor receptor inhibitor ZD1839
Zanchi, Chiara, et al (2005) Cancer Res, 65:2364-72 (2005)
Species Reactivity Key Applications Host Format Antibody Type
H ELISA, WB Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-EGFR (Tyr1086) Antibody, clone 8B8
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes phosphorylated EGFR on Tyrosine 1086.
Molecular Weight:
180 kDa
Immunogen:
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Tyr 1086 in human EGFR.
Modifications:
Phosphorylation
Clone:
8B8
Isotype:
IgG1
Species Reactivity:
Human
Control:
Includes pervanadate treated A431 cell lysate as a positive control
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Subsequent thiophilic adsorption and size exclusion chromatography
Storage Conditions:
2 years at -20°C from date of shipment
UniProt Number:
P00533
Entrez Gene Number:
NM_005228.3
NM_201282.1
NM_201283.1
NM_201284.1
Gene Symbol:
EGFR
ERBB1
ERBB
mENA
Alternate Names:
ERBB; ERBB1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
ELISA
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
SIZE: 1210 amino acids; 134277 Da
SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of t |