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Anti-HePTP (PTPN7), clone D24 Antibody (04-278)
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REFERENCES
Haematopoietic protein tyrosine phosphatase (HePTP) phosphorylation by cAMP-dependent protein kinase in T-cells: dynamics and subcellular location.
Nika, Konstantina, et al. (2004) Biochem. J., 378: 335-42 (2004)
Structure of the hematopoietic tyrosine phosphatase (HePTP) catalytic domain: structure of a KIM phosphatase with phosphate bound at the active site.
Mustelin, Tomas, et al. (2005) J. Mol. Biol., 354: 150-63 (2005)
Negative regulation of T cell antigen receptor signal transduction by hematopoietic tyrosine phosphatase (HePTP).
Saxena, M, et al. (1998) J. Biol. Chem., 273: 15340-4 (1998)
Species Reactivity Key Applications Host Format Antibody Type
H WB Mouse Purified Monoclonal Antibody
Description:
Anti-HePTP (PTPN7) Antibody, clone D24
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes HePTP (PTPN7) at ~38-40 kDa.
Molecular Weight:
38-40 kDa
Immunogen:
Full length recombinant His-tag protein.
Clone:
D24
Isotype:
IgG2bκ
Species Reactivity:
Human
Application Notes:
Western Blot Analysis: 0.5-2 µg/mL of this lot was used to detect HePTP(PTPN7) in Jurkat cell lysates.
Control:
Jurkat cell lysate
Quality Assurance:
Routinely evaluated by Western Blot on Jurkat cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG2bκ in buffer containing PBS with 0.1% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P35236
Entrez Gene Number:
NP_002823
Gene Symbol:
PTPN7
HePTP
LPTP
PTPNI
BPTP-4
LC-PTP
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Alternate Names:
Protein tyrosine phosphatase, non-receptor type 7
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This gene is preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells. The noncatalytic N-terminus of this PTP can interact with MAP kinases and suppress the MAP kinase activities. This PTP was shown to be involved in the regulation of T cell antigen receptor (TCR) signaling, which was thought to function through dephosphorylating the molecules related to MAP kinase pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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UniProt Summary:
FUNCTION: Protein phosphatase that acts preferentially on tyrosine-phosphorylated MAPK1. Plays a role in the regulation of T and B-lymphocyte development and signal transduction.
CATALYTIC ACTIVITY: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
SUBUNIT: Monomer. Interacts with MAPK1, MAPK3 and several other MAP kinases.
SUBCELLULAR LOCATION: Cy |