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Anti-phospho-EGFR (Thr669) Antibody, clone 5F10
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REFERENCES
Epidermal growth factor receptor threonine and serine residues phosphorylated in vivo
Heisermann, G J and Gill, G N (1988) J Biol Chem, 263:13152-8 (1988)
Mutational removal of the Thr669 and Ser671 phosphorylation sites alters substrate specificity and ligand-induced internalization of the epidermal growth factor receptor
Heisermann, G J, et al (1990) J Biol Chem, 265:12820-7 (1990)
Cisplatin induces PKB/Akt activation and p38(MAPK) phosphorylation of the EGF receptor
Winograd-Katz, S E and Levitzki, A (2006) Oncogene, 25:7381-90 (2006)
Species Reactivity Key Applications Host Format Antibody Type
H WB Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-EGFR (Thr669) Antibody, clone 5F10
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes EGFR when phosphorylated at Threonine 669.
Molecular Weight:
180 kDa
Immunogen:
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 669 in human EGFR.
Modifications:
Phosphorylation
Clone:
5F10
Isotype:
IgG1
Species Reactivity:
Human
Control:
Includes PMA and pervanadate treated A431 cell lysate as a positive control
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Thiophilic adsoption and size exclusion chromatography
Presentation:
100 µg of mouse monoclonal IgG1 liquid in 0.01 mg/ml PBS containing 0.09% sodium azide, PEG, and sucrose with 50 % glycerol.
Storage Conditions:
6 months at -20°C from date of shipment
UniProt Number:
P00533
Entrez Gene Number:
NM_005228.3
NM_201282.1
NM_201283.1
NM_201284.1
Gene Symbol:
EGFR
ERBB1
ERBB
mENA
Alternate Names:
ERBB, ERBB1, epidermal growth factor receptor
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
SIZE: 1210 amino acids; 134277 Da
SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
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