|
Anti-19S regulator non-ATPase subunit S14/Rpn12 Antibody, clone p31-27
Close
REFERENCES
Subunit arrangement in the human 20S proteasome
Kopp, F, et al (1997) Proc Natl Acad Sci USA, 94:2939-44 (1997)
The 26S proteasome: subunits and functions
Tanaka, K and Tsurumi, C (1997) Mol Biol Rep, 24:3-11 (1997)
Species Reactivity Key Applications Host Format Antibody Type
H WB Mouse Purified Monoclonal Antibody
Description:
Anti-19S regulator non-ATPase subunit S14/Rpn12 Antibody, clone p31-27
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes 19S regulator non-ATPase subunit S14/Rpn12.
Molecular Weight:
30 kDa
Immunogen:
Recombinant human Rpn12 protein.
Clone:
p31-27
Isotype:
IgG1
Species Reactivity:
Human
Presentation:
100 µL of purified immunoglobulin in PBS containing 0.01M sodium azide. Dilute to working strength with phosphate buffered saline pH 7.2-7.4.
Storage Conditions:
Stable for 1 year at -20°C from date of shipment for up to 12 months.
UniProt Number:
P48556
Entrez Gene Number:
NM_002812.3
Gene Symbol:
PSMD8
Nin1p
HYPF
HIP6
S14
MGC1660
p31
View All »
Alternate Names:
S14; p31
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 1.
View All »
UniProt Summary:
FUNCTION: SwissProt: P48556 # Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Necessary for activation of the CDC28 kinase.
SIZE: 257 amino acids; 30005 Da
SIMILARITY: SwissProt: P48556 ## Belongs to the proteasome subunit S14 family.
View All »
Brand Family:
Upstate
Product Name:
Anti-19S regulator non-ATPase subunit S14/Rpn12 Antibody, clone p31-27
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µL
Format:
Purified
Host:
Mouse
Applications:
Anti-19S regulator non-ATPase subunit S14/Rpn12 Antibody, clone p31-27 detects level of 19S regulator non-ATPase subunit S14/Rpn12 & has been published & validated for use in WB. |