|
Anti-19S regulator non-ATPase subunit S5a/Rpn10 Antibody, clone S5a-18
Close
REFERENCES
Purification of poly-ubiquitinated proteins by S5a-affinity chromatography
Layfield, R, et al (2001) Proteomics, 1:773-7 (2001)
Proteins containing the UBA domain are able to bind to multi-ubiquitin chains
Wilkinson, C R, et al (2001) Nat Cell Biol, 3:939-43 (2001)
Structural studies of the interaction between ubiquitin family proteins and proteasome subunit S5a
Walters, Kylie J, et al (2002) Biochemistry, 41:1767-77 (2002)
Species Reactivity Key Applications Host Format Antibody Type
H IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-19S regulator non-ATPase subunit S5a/Rpn10 Antibody, clone S5a-18
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Product Overview:
Rpn10 (S5a) is a non-ATPase subunit of the 19S regulatory complex of the 26s proteasome. Subunit Rpn10 (S5a) of the 26S proteasome was originally identified as a protein capable of binding poly-ubiquitin chains of four or more ubiquitins, in the absence of other 26S proteasome subunits.
Specificity:
Recognizes 19S regulator non-ATPase subunit S5a/Rpn10.
Molecular Weight:
41 kDa
Immunogen:
Full length human recombinant Rpn10 protein.
Clone:
S5a-18
Isotype:
IgG1
Species Reactivity:
Human
Presentation:
100 µL of purified immunoglobulin in PBS containing 0.01M sodium azide. Dilute to working strength with phosphate buffered saline pH 7.2-7.4.
Storage Conditions:
Stable for 1 year at -20°C from date of shipment for up to 12 months.
UniProt Number:
P55036
Entrez Gene Number:
NM_153822.2
NM_002810.2
Gene Symbol:
PSMD4
pUB-R5
OTTHUMP00000059963
Rpn10
AF-1
AF
MCB1
S5A
ASF
View All »
Alternate Names:
S5a
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21.
View All »
UniProt Summary:
FUNCTION: SwissProt: P55036 # Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion.
SIZE: 377 amino acids; 40737 Da
SUBUNIT: The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or bot |