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Anti-p16/INK4a, clone EP435Y
Species Reactivity Key Applications Host Format Antibody Type
H WB, FC, IP Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-p16/INK4a Antibody, clone EP435Y, rabbit monoclonal
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Specificity:
This antibody recognizes p16/INK4a.
Molecular Weight:
16 kDa
Immunogen:
Recombinant p16/INK4a human protein
Clone:
EP435Y
Isotype:
IgG
Background Information:
p16 (INK4a, CDKN2A, cyclin-dependent kinase inhibitor 2A) is a tumor suppressor gene that is important in regulating cell cycle. Mutations in p16 can result in various cancers, most notably melanoma. p16 is a splice variant that comes from the gene CDKN2A. CDKN2A gene produces 2 major proteins, p16 (INK4) and p14 (ARF), which binds MDM2.
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Species Reactivity:
Human
Species Reactivity Note:
Proven to react with human. Not expected to react with mouse and rat
Application Notes:
Immunoprecipitation: A 1:50 dilution of a previous lot was used in IP.
Flow Cytometry: A 1:10 dilution of a previous lot was used in FC.
Control:
HEK293 whole cell lysate.
Quality Assurance:
Routinely evaluated by Western Blot on HEK293 whole cell lysate.
Western Blot Analysis: A 1:500-1:2,000 dilution of this lot was used to detect p16/INK4a HEK293 whole cell lysate.
Purification Method:
Unpurified
Presentation:
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P42771
Entrez Gene Number:
NM_000077.3
Gene Symbol:
ARF
CDK4I
CDKN2
CMM2
INK4
INK4a
MLM
MTS-1
MTS1
P16
TP16
p14
p14ARF
p16
p16-INK4
p16-INK4a
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Alternate Names:
CDK4 inhibitor p16-INK4
Cyclin-dependent kinase 4 inhibitor A
Multiple tumor suppressor 1
cell cycle negative regulator beta
cyclin-dependent kinase inhibitor 2A
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
cyclin-dependent kinase inhibitor p16
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Flow Cytometry
Immunoprecipitation
Entrez Gene Summary:
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; |