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Anti-p27(Kip1), clone Y236
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Species Reactivity Key Applications Host Format Antibody Type
H, R WB, FC, IP, ICC, IHC Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-p27(Kip1) Antibody, clone Y236, rabbit monoclonal
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Specificity:
This antibody recognizes p27(Kip1) at the C-terminus.
Molecular Weight:
27 kDa
Epitope:
C-terminus
Immunogen:
Synthetic peptide corresponding to the C-terminus of human p27 (Kip1).
Clone:
Y236
Isotype:
IgG
Background Information:
p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4. p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1); while phosphorylation by hKIS on Serine 10 signals the nuclear export to the cytoplasm.
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Species Reactivity:
Human
Rat
Species Reactivity Note:
Proven to react with Human and Rat. Not expected to react with Mouse.
Application Notes:
Immunocytochemistry: A 1:100-250 dilution of a previous lot was used in IC.
Immunohistochemistry: A 1:100-250 dilution of a previous lot was used in IH.
Immunoprecipitation: A 1:50 dilution of a previous lot was used in IP.
Flow Cytometry: A 1:40 dilution of a previous lot was used in FC.
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Control:
MCF-7 cell lysate.
Quality Assurance:
Routinely evaluated by western blot on MCF-7 cell lysate.
Western Blot Analysis: A 1:1,000-1:2,000 dilution of this lot was used to detect p27(Kip1) in MCF-7 cell lysate.
Purification Method:
Unpurified
Presentation:
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P46527
Entrez Gene Number:
NP_004055.1
Gene Symbol:
CDKN4
KIP1
MEN1B
MEN4
P27KIP1
p27Kip1
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Alternate Names:
Cyclin-dependent kinase inhibitor
Cyclin-dependent kinase inhibitor p27
cyclin-dependent kinase inhibitor 1B
cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Flow Cytometry
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
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Entrez Gene Summary:
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encod |