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Anti-Nibrin/NBS1, clone Y112
Species Reactivity Key Applications Host Format Antibody Type
H, M ICC, IHC, IH(P), IP, WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-Nibrin/NBS1 Antibody, clone Y112, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes Nibrin/NBS1.
Molecular Weight:
95 kDa
Epitope:
C-terminus
Immunogen:
KLH conjugated to Nibrin/NBS1 linear peptide.
Clone:
Y112
Isotype:
IgG
Background Information:
Nibrin (NBS1, NBN) is known to be a key regulator of DNA Damage repair by forming a complex with Rad50 and MRE11. This complex, more commonly referred to as MRN (Mre11/Rad50/Nibrin) is important in double-stranded break repair, DNA recombination, cell cycle checkpoint control, and meiosis. This complex quickly recognizes double stranded break (DSB) sites and quickly relocates to these sites. Nibrin senses DNA damage by recruiting ATM, ATR, and DNA-PK to the damage sites and activation their functions.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Proven to react with human and mouse. Not expected to react with rat.
Application Notes:
Immunohistochemistry: A 1:50 dilution of a previous lot was used in IH.
Immunoprecipitation: A 1:60 dilution of a previous lot was used in IP.
Immunocytochemistry: A 1:50 dilution of a previous lot was used in IC.
Control:
HeLa whole cell lysate.
Quality Assurance:
Routinely evaluated by Western Blot on HeLa whole cell lysate.
Western Blot Analysis: A 1:250 dilution of this lot was used to detect Nibrin/NBS1 in HeLa whole cell lysate.
Purification Method:
Unpurified
Presentation:
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
O60934
Entrez Gene Number:
NM_002485.4
Gene Symbol:
AT-V1
AT-V2
ATV
FLJ10155
MGC87362
NBS
NBS1
P95
nibrin
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Alternate Names:
Cell cycle regulatory protein p95
Nijmegen breakage syndrome 1 (nibrin)
Nijmegen breakage syndrome protein 1
p95 protein of the MRE11/RAD50 complex
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunocytochemistry
Immunohistochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting
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Entrez Gene Summary:
Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, |