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Anti-NFĸB, p65, clone E379
Species Reactivity Key Applications Host Format Antibody Type
H FC, ICC, IHC, IH(P), IP, WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-NFĸB Antibody, p65, clone E379, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes NFκB p65.
Molecular Weight:
65 kDa
Epitope:
C-Terminus
Immunogen:
KLH conjugated to NFκB p65 linear peptide
Clone:
E379
Isotype:
IgG
Background Information:
The transcription factor NFĸB (Nuclear Factor kappa B) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response. NFĸB represents a group of 5 structurally related and evolutionarily conserved proteins in mammals that include Rel (cRel), RelA (p65), RelB, NFκB1 (p50 and its precursor p105), and NFκB2 (p52 and its precursor p100). NFκB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes, though the p50/p65 heterodimer of NFκB appears to be the most abundant in cells. A critical component in NFκB regulation is the IκB Kinase (IKK) complex. NFκB transcription factors exist in their inactive form and are retained in the cytoplasm by the bound inhibitory IκB proteins. Upon stimulation by multiple inducers including viruses or cytokines, IκB is rapidly phosphorylated and degraded, resulting in the release of the NFκB complex. The p105 subunit is cleaved into its active p50 form. This cleavage exposes the NLS sequence on the p50 subunit. The p50/p65 heteroduplex then translocates to the nucleus where it activates gene transcription. The wide variety of genes regulated by NFκB includes those encoding cytokines, chemokines, adhesion molecules, acute phase proteins and inducible effector enzymes.
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Species Reactivity:
Human
Species Reactivity Note:
Proven to react with human. Not expected to react with mouse or rat.
Application Notes:
Immunocytochemistry: A 1:250-500 dilution of a previous lot was used in IC.
Immunohistochemistry: A 1:250-500 dilution of a previous lot was used in IH.
Immunoprecipitation: A 1:200 dilution of a previous lot was used in IP.
Flow Cytometry: A 1:100 dilution of a previous lot was used in FC.
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Quality Assurance:
Routinely evaluated by Western Blot on HeLa whole cell lysate.
Western Blot Analysis: A 1:50,000 dilution of this lot was used to detect NFκB p65 in HeLa whole cell lysate.
Purification Method:
Unpurified
Presentation:
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage
UniProt Number:
P19838
Entrez Gene Number:
NM_021975.2
Gene Symbol:
NFKB
Alternate Names:
Nuclear Factor kappa B
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic |