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Anti-NFĸB p50, clone E381
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REFERENCES
Dehydroxymethylepoxyquinomicin inhibits expression and production of inflammatory mediators in interleukin-1beta-induced human chondrocytes.
Venera Cardile,Giuseppina Frasca,Massimo Libra,Silvia Caggia,Kazuo Umezawa,Annamaria Panico,Grazia Malaponte (2010) Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology.25
Species Reactivity Key Applications Host Format Antibody Type
H, M, R FC, ICC, IHC, IH(P), WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-NFĸB p50 Antibody, clone E381, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes NFκB p50 and its p105 uncleaved precursor.
Molecular Weight:
105/50 kDa
Immunogen:
KLH conjugated to NFB p105/50 linear peptide.
Clone:
E381
Isotype:
IgG
Background Information:
The transcription factor NFĸB (Nuclear Factor kappa B) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response. NFĸB family represents a group of 5 structurally related and evolutionarily conserved proteins in mammals that include Rel (cRel), RelA (p65), RelB, NFĸB1 (p50 and its precursor p105), and NFĸB2 (p52 and its precursor p100). NFĸB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes, though the p50/p65 heterodimer of NFĸB appears to be the most abundant in cells. A critical component in NFĸB regulation is the IĸB Kinase (IKK) complex. NFĸB transcription factors exist in their inactive form and are retained in the cytoplasm by the bound inhibitory IĸB proteins. Upon stimulation, IĸB is rapidly phosphorylated and degraded, resulting in the release of the NFĸB complex. The p105 subunit is cleaved into its active p50 form. This cleavage exposes the NLS sequence on the p50 subunit. p50/p65 then translocates to the nucleus where it activates gene transcription. The wide variety of genes regulated by NFĸB includes those encoding cytokines, chemokines, adhesion molecules, acute phase proteins and inducible effector enzymes.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Proven to react with human, mouse and rat.
Application Notes:
Immunocytochemistry: A 1:250-500 dilution of a previous lot was used in IC.
Immunohistochemistry: A 1:250-500 dilution of a previous lot was used in IH.
Flow Cytometry: A 1:30 dilution of a previous lot was used in FC.
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Quality Assurance:
Routinely evaluated by Western Blot on HeLa whole cell lysate.
Western Blot Analysis: A 1:250-1:1,000 dilution of this lot was used to detect NFĸB p50 in HeLa whole cell lysate.
Purification Method:
Unpurified
Presentation:
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
UniProt Number:
P19838
Entrez Gene Number:
NM_003998.2
Gene Symbol:
NFKB
Alternate Names:
Nuclear Factor kappa B
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