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Anti-HDAC4, clone Y417, rabbit monoclonal Antibody (04-232)
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REFERENCES
HDAC4 regulates neuronal survival in normal and diseased retinas.
Chen, Bo and Cepko, Constance L (2009) Science, 323: 256-9 (2009)
Histone deacetylase 4 is required for TGFbeta1-induced myofibroblastic differentiation.
Glenisson, Wendy, et al. (2007) Biochim. Biophys. Acta, 1773: 1572-82 (2007)
Crystal structure of a conserved N-terminal domain of histone deacetylase 4 reveals functional insights into glutamine-rich domains.
Guo, Liang, et al. (2007) Proc. Natl. Acad. Sci. U.S.A., 104: 4297-302 (2007)
Species Reactivity Key Applications Host Format Antibody Type
H, not M, not R FC, IP, WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-HDAC4 Antibody, clone Y417, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes Histone Deacetylase 4 at the C-terminus.
Molecular Weight:
140 kDa
Epitope:
C-terminus
Immunogen:
KLH conjugated to a synthetic peptide from the C-terminal region of human Histone Deacetylase 4.
Clone:
Y417
Isotype:
IgG
Background Information:
Histone deacetylation acts a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC4 is a histone deacetylase which deacetylates lysines on the core histones. HDAC4 accumulates in the nuclei of myotubes, suggesting involvement in muscle differentiation. HDAC4 does not bind DNA directly but interacts with the myocyte enhancer factors Mef2a, Mef2c, and Mef2d. HDAC4 also interacts with the breast cancer-associated transcriptional repressor PLU-1/JARID1B, the Nr2c1/TR2 nuclear receptor, as well as the 14-3-3 chaperone protein, in a phosphorylation dependent manner. It forms part of a multi-protein complex with RbAp48 and HDAC3.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with human HDAC4. Not expected to react with mouse and rat HDAC4.
Non-Reactive Species:
Mouse
Rat
Application Notes:
Flow Cytometry:
1:30 dilution of a previous lot was used for flow cytometry.
Immunoprecipitation:
1:50 dilution of a previous lot was used for immunoprecipitation.
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Control:
HeLa whole cell lysate.
Quality Assurance:
Routinely evaluated by Western Blot on HeLa whole cell lysate.
Western Blot Analysis:
A 1:500 dilution of this lot was used to detect Histone Deacetylase 4 on HeLa whole cell lysate.
Purification Method:
Unpurified
Presentation:
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P56524
Entrez Gene Number:
NM_006037
Gene Symbol:
EC 3.5.1.98
HA6116
HD4
HDAC-A
HDACA
KIAA0288
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Alternate Names:
histone deacetylase 4
histone deacetylase A
Usage Statement:
Unless oth |