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Anti-HDAC2, clone Y461, rabbit monoclonal Antibody (04-229)
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REFERENCES
HDAC2 blockade by nitric oxide and histone deacetylase inhibitors reveals a common target in Duchenne muscular dystrophy treatment.
Colussi, Claudia, et al. (2008) Proc. Natl. Acad. Sci. U.S.A., 105: 19183-7 (2008)
HDAC2 deficiency and histone acetylation.
Ree, Anne Hansen, et al. (2008) Nat. Genet., 40: 812-3; author reply 813 (2008)
S-Nitrosylation of histone deacetylase 2 induces chromatin remodelling in neurons.
Nott, Alexi, et al. (2008) Nature, 455: 411-5 (2008)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R FC, ICC, IHC, IH(P), IP, WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-HDAC2 Antibody, clone Y461, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes Histone Deacetylase 2 at the C-terminus.
Molecular Weight:
55 kDa
Epitope:
C-terminus
Immunogen:
KLH conjugated to a synthetic peptide from the C-terminal region of human Histone Deacetylase 2.
Clone:
Y461
Isotype:
IgG
Background Information:
Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Demonstrated to react with human, mouse and rat HDAC2.
Application Notes:
Flow Cytometry:
1:60 dilution of a previous lot was used on flow cytometry.
Immunoprecipitation:
1:60 dilution of a previous lot was used on immunoprecipitation.
Immunocytochemistry:
1:250-500 dilution of a previous lot was used in immunocytochemistry.
Immunohistochemistry:
1:250-500 dilution of a previous lot was used in immunohistochemistry.
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Control:
K562 whole cell lysate.
Quality Assurance:
Routinely evaluated by Western Blot on K562 whole cell lysate.
Western Blot Analysis:
A 1:2000-1:10,000 dilution of this lot was used to detect Histone Deacetylase 2 on K562 whole cell lysate.
Purification Method:
Unpurified
Presentation:
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
Q92769
Entrez Gene Number:
NM_001527
Gene Symbol:
EC 3.5.1.98
HD2
OTTHUMP00000040427
RPD3
YAF1
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Alternate Names:
YY1-associated factor 1
histone deacetylase 2
transcriptional regulator homolog RPD3
Usage Statement:
Unless otherwise stated in our catalog or other |