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Anti-Estrogen Receptor α, clone E115 Antibody
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, ICC, IH(P) Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-Estrogen Receptor α Antibody, clone E115, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes Estrogen Receptor α.
Molecular Weight:
67 kDa
Immunogen:
Synthetic peptide directed against human Estrogen Receptor α.
Clone:
E115
Isotype:
IgG
Background Information:
Estrogen Receptor α (ER alpha) is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Proven to react with human, mouse and rat.
Application Notes:
Immunohistochemistry:
Immunocytochemistry:
1:100 dilution of a previous lot was used on immunocytochemistry.
Control:
MCF-7 cell lysate.
Quality Assurance:
Routinely evaluated by western blot on MCF-7 cell lysate.
Western Blot Analysis:
A 1:250-1:500 dilution of this lot was used to detect Estrogen Receptor in MCF-7 cell lysate.
Purification Method:
Unpurified
Presentation:
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P03372
Entrez Gene Number:
NP_000116.2
Gene Symbol:
ER
ER-alpha
ESR
ESRA
Era
NR3A1
DKFZp686N23123
OTTHUMP00000017718
OTTHUMP00000017719
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Alternate Names:
Estradiol receptor
Nuclear receptor subfamily 3 group A member 1
estrogen receptor 1
estrogen receptor alpha
oestrogen receptor
steroid hormone receptor
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffi |