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Anti-Elk1, (C-term), clone E277, rabbit monoclonal Antibody
Species Reactivity Key Applications Host Format Antibody Type
H ICC, IHC, IH(P), IP, WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-Elk1 Antibody, (CT), clone E277, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes Elk-1 at the C-Terminus. The antibody does not cross-react with other ETS family members.
Molecular Weight:
47-60 kDa Molecular weight varies depending on modifications.
Epitope:
C-terminus
Immunogen:
KLH conjugated linear peptide of Elk-1.
Clone:
E277
Isotype:
IgG
Background Information:
ELK1 is a transcription factor and a member of the Ets family of transcription factors. Elk1 is a downstream transcription factor of the Ras-Raf-MEK-Erk (canonical MAPK) pathway.
Species Reactivity:
Human
Species Reactivity Note:
Proven to react with human. Not expected to react with mouse and rat.
Application Notes:
Immunohistochemistry: 1:50 dilution from a previous lot was used on immunohistochemistry.
Immunocytochemistry: 1:100 dilution from a previous lot was used on immunocytochemistry.
Immunoprecipitation: 1:80 dilution from a previous lot was used on immunoprecipitation.
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Control:
A431 whole cell lysate.
Quality Assurance:
Routinely evaluated by Western Blot on A431 whole cell lysate.
Western Blot Analysis:
A 1:500 dilution of this lot was used to detect Elk-1 on A431 whole cell lysate.
Presentation:
Unpurified rabbit monoclonal IgG in buffer contaiing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P19419
Entrez Gene Number:
NM_005229
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunocytochemistry
Immunohistochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting
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Entrez Gene Summary:
This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
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UniProt Summary:
Function: Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of th |