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Anti-Cyclin E1,clone EP435E rabbit monoclonal Antibody
Species Reactivity Key Applications Host Format Antibody Type
H, not M, not R FC, ICC, IP, WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-Cyclin E1 Antibody,clone EP435E rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes Cyclin E1.
Molecular Weight:
50 kDa
Epitope:
Internal
Immunogen:
Synthetic peptide identical to an internal region of human Cyclin E1.
Clone:
EP435E
Isotype:
IgG
Background Information:
Cyclins are, as their name suggests, expressed at specific times during the cell cycle, and play a crucial role in the regulation of the cell cycle. Cyclins associate with cyclin-dependent kinases (cdks), and the association is absolutely required for cdk activity. At the conclusion of the relevant phase of the cell cycle, cyclins are rapidly ubiquitinated and degraded, thus returning cdks to their inactive state. Cyclin A associates with cdk2, and together with cyclin E is involved in the G1/S transition. Cyclin A/cdk2 remains active during S-phase. Cyclin B associates with cdk1/cdc2 to regulate the G2/M boundary. The D-cyclins bind cdk4 and cdk6, and result in Rb phosphorylation. The cyclin D1 gene was found in chronic B cell lymphocytic leukemia, and was designated Bcl-1 in a previously used nomenclature.
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Species Reactivity:
Human
Species Reactivity Note:
Proven to react with human. Not expected to react with rat or mouse.
Non-Reactive Species:
Mouse
Rat
Application Notes:
Immunocytochemistry: 1:100-250 dilution of a previous lot was used on immunocytochemistry.
Immunoprecipitation: 1:80 dilution of a previous lot was used on immunoprecipitation.
Flow Cytometry: 1:20 dilution of a previous lot was used on flow cytometry.
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Control:
HeLa whole cell lysate.
Quality Assurance:
Routinely evaluated by Western Blot on HeLa whole cell lysate.
Western Blot Analysis:
A 1:1000-1:2000 dilution of this lot was used to detect Cyclin E1 on HeLa whole cell lysate.
Purification Method:
Unpurified
Presentation:
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P24864
Entrez Gene Number:
NM_001238.1
Gene Symbol:
CCNE
Alternate Names:
cyclin E1
cyclin Es
cyclin Et
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Immunocytochemistry
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
The protein encoded |