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Anti-Cyclin D1, clone EP272Y, rabbit monoclonal Antibody
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R IP, WB, ICC, IHC Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-Cyclin D1 Antibody, clone EP272Y, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes Cyclin D1.
Molecular Weight:
36 kDa
Epitope:
N-terminus
Immunogen:
KLH conjugated to Cyclin D1 linear peptide.
Clone:
EP272Y
Isotype:
IgG
Background Information:
Cyclins are, as their name suggests, expressed at specific times during the cell cycle, and play a crucial role in the regulation of the cell cycle. Cyclins associate with cyclin-dependent kinases (cdks), and the association is absolutely required for cdk activity. At the conclusion of the relevant phase of the cell cycle, cyclins are rapidly ubiquitinated and degraded, thus returning cdks to their inactive state. Cyclin A associates with cdk2, and together with cyclin E is involved in the G1/S transition. Cyclin A/cdk2 remains active during S-phase. Cyclin B associates with cdk1/cdc2 to regulate the G2/M boundary. The D-cyclins bind cdk4 and cdk6, and result in Rb phosphorylation. The cyclin D1 gene was found in chronic B cell lymphocytic leukemia, and was designated Bcl-1 in a previously used nomenclature.
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Species Reactivity:
Human
Mouse
Rat
Application Notes:
Immunocytochemistry: 1:50-100 dilution from a previous lot was used in immunocytochemistry.
Immunoprecipitation: 1:50 dilution from a previous lot was used in immunoprecipitation.
Control:
MCF-7 cell lysate.
Quality Assurance:
Routinely evaluated by western blot on MCF-7 cell lysate.
Western Blot Analysis:
A 1:1,000 dilution of this lot was used to detect Cyclin D1 on MCF-7 cell lysate.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P24385
Entrez Gene Number:
NP_444284.1
Gene Symbol:
BCL1
D11S287E
PRAD1
U21B31
Alternate Names:
B-cell CLL/lymphoma 1
BCL-1 oncogene
G1/S-specific cyclin D1
PRAD1 oncogene
cyclin D1
cyclin D1 (PRAD1: parathyroid adenomatosis 1)
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Immunocytochemistry
Immunohistochemistry
Entrez Gene Summary:
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Differ |