This lectin has a molecular weight of 40,000-46,000 and is composed of four subunits. There appear to be two different chains in combinations giving rise to five isolectins. The isoelectric point of this lectin is pH 4.7, and it is probably devoid of carbohydrate or metal ions. The carbohydrate specificity of either type of subunit is likely to be the same, preferring alpha linked N-acetylgalactosamine structures. Fluorescein labeled Maclura Pomifera Lectin is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled Maclura Pomifera Lectin has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Fluorescein labeled Maclura Pomifera Lectin has an excitation at 495 nm and an emission at about 515 nm. |