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This lectin is a 116,000 to 125,000 molecular weight glycoprotein with an isoelectric point of pH 5.4. Two subunits of about 60,000 daltons are produced in SDS gels, dissociating into 4 chains of about 30,000 daltons in the presence of reducing agents. The binding specificity is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked alpha or beta to the 3 or 6 position of galactose. Used to fractionate lymphocyte populations, the lectin although not mitogenic, elicits the production of lymphokines from murine splenocytes (references available upon request).
Fluorescein labeled Wisteria Floribunda Lectin is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled Wisteria Floribunda Lectin has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Fluorescein labeled Wisteria Floribunda Lectin has an excitation at 495 nm and an emission at about 515 nm. |