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This glycoprotein of about 140,000 daltons consists of six closely related lectins with isoelectric points between pH 4.3 to pH 4.7. Most of the 35,000 dalton subunits appear to consist of two disulfide linked chains of about 17,000 daltons. This lectin has a carbohydrate binding specificity toward type 1 or type 2 chain blood group B structures but will bind other oligosaccharides containing galactosyl (a-1,3) galactose. Unlike Ulex europaeus and Lotus tetragonolobus lectins, EEL has a high affinity toward type 1 chain blood group H structures. This lectin has been reported to bind to endothelial cells from human and non-human sources and recognizes carbohydrate structures on the surface of stimulated murine peritoneal lymphoid cells.
Fluorescein labeled Euonymus Europaeus Lectin is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled Euonymus Europaeus Lectin has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Fluorescein labeled Euonymus Europaeus Lectin has an excitation at 495 nm and an emission at about 515 nm. |