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14-197 | Casein Kinase 2 Protein, active, 10 μg
Active, recombinant full-length human Casein Kinase 2 protein, containing an α-subunit N-terminal 6His tag & a β-subunit N-terminal GST tag. For use in Kinase Assays.
Description
Catalogue Number 14-197
Brand Family Upstate
Trade Name
Upstate
Description Casein Kinase 2 Protein, active, 10 μg
Overview Recombinant full-length human Casein Kinase 2 protein, containing an &alpyha;-subunit N-terminal 6His tag and a β-subunit N-terminal GST tag.
Product Information
Presentation 20mM MOPS, pH 7.4, 1mM EDTA, 0.1% (v/v) Triton X-100, 0.3% 2-mercaptoethanol, 1mM benzamidine, 50% glycerol.
Applications
Application Active, recombinant full-length human Casein Kinase 2 protein, containing an α-subunit N-terminal 6His tag & a β-subunit N-terminal GST tag. For use in Kinase Assays.
Key Applications
Kinase Assay
Biological Information
Source Expressed in Sf21 insect cells.
Species Human
Specific Activity For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
NM_177560.2
NM_001895.3
NM_177559.2
Entrez Gene Summary Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene.
Gene Symbol
CKII
CSNK2A1
CK2A1
Protein Target CK2
Purification Method Sequential chromatography using heparin Sepharose™ and Mono Q anion exchange resin
Target Sub-Family CMGC
UniProt Number
P68400
UniProt Summary FUNCTION: SwissProt: P68400 # Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.
SIZE: 391 amino acids; 45144 Da
SUBUNIT: Tetramer composed of an alpha chain, an alpha' and two beta chains. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Interacts with RNPS1.
SIMILARITY: SwissProt: P68400 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. & Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance Routinely evaluated by phosphorylation of the Casein Kinase 2 Substrate Peptide.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 1 year at -20°C.
Packaging Information
Material Size 10 µg
Material Package 2 vials at 5μg each
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-197M when ord |