|
产品编号 P8811
CAS号 9036-06-0
蛋白酶 来源于灰色链霉菌
Protease from Streptomyces griseus
powder, BioReagent, suitable for mouse embryo cell culture, ≥3.5 units/mg solid
别名: Actinase E Pronase E
产品名称
Other Notes
An unusually non-specific protease.
Preparation Note
Collected from culture broth of S. griseus and purified by successive column procedures.
Quality
Contains approximately 25% calcium acetate.
Specificity
A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.
Unit Definition
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Application
Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.
This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.
Physical properties
Completely inactivated by heating above 80 °C for 15-20 minutes.
product line BioReagent
form powder
impurities starch, essentially free
suitability suitable for mouse embryo cell culture
storage temp. −20°C |