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产品编号 D9542
CAS号 28718-90-3
4′,6-Diamidino-2-phenylindole dihydrochloride
powder, ≥98% (HPLC and TLC), suitable for fluorescence
别名: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride DAPI dihydrochloride
经验分子式（希尔表示法） C16H15N5 · 2HCl
分子量 350.25
产品名称
Frequently Asked Questions
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Live Chat and Frequently Asked Questions are available for this Product.
Biochem/physiol Actions
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Caution
Protect from light.
Application
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
Suitable for
• DNA staining in agarose gels
• analysis of changes in DNA during apoptosis
• detection of mycoplasma
• photofootprinting of DNA
• immunofluorescent staining of cells
General description
DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) is a cell permeable, fluorescent dye that binds to DNA.
性质
Related Categories Apoptosis Detection, Apoptosis and Cell Cycle, Cell Biology, Cell Signaling and Neuroscience, Fluorescent,
Quality Level PREMIUM
grade for molecular biology
assay ≥98% (HPLC and TLC)
form powder
solubility H2O: soluble20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.)
PBS: insoluble
absorption ε1mM/263 nm, H2O 30
fluorescence λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
suitability suitable for fluorescence
storage temp. 2-8°C |