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产品编号 B6149
CAS号 102185-33-1
5-溴-4-氯-3-吲哚磷酸 二钠盐
5-Bromo-4-chloro-3-indolyl phosphate disodium salt
别名: BCIP® X-phosphate disodium salt
线性分子式 C8H4BrClNO4P · 2Na
分子量 370.43
Substrates
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
assay ≥98% (HPLC)
solubility H2O: soluble20 mg/mL
DMF: insoluble
storage temp. −20°C |