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TWEAK Receptor, human recombinant
Activates cell apoptotic machinery
Product Overview
Product Name: TWEAK Receptor, human recombinant
Alternate Name/Synonyms: TNF-related weak inducer of apoptosis receptor, TNFRSF12A, FGF-inducible 14, TWEAKR
Gene Symbol: TNFRSF12A
Accession #: Q9NP84
Gene ID: 51330
Source: E. Coli
Appearance: Lyophilized powder
Physical Form Description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5 and 50 mM NaCl.
Molecular Weight: 5.6 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Biological Activity: Determined by its ability to inhibit TWEAK-induced weak cell death of HT29 cells. The expected ED50 for this effect is 1.0-3.0 μg/ml.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: TWEAKR belongs to the TNFR superfamily of transmembrane proteins and contains a cytoplasmic “death domain,” which can activated the cell’s apoptotic machinery. It is expressed in the spleen, thymus, peripheral blood lymphocytes, colon, and small intestine. Signal transduction by TWEAKR can be activated by either the membrane anchored or the soluble TWEAK. Recombinant soluble TWEAKR is a 53 amino acid polypeptide (5.6 kDa) comprising the entire extracellular domain of the full length TWEAKR protein.
Amino Acid Sequence: EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |