TSG, human recombinant Inhibits BMP activity by binding directly to BMP proteins Product Overview Product Name: TSG, human recombinant Alternate Name/Synonyms: Twisted Gastrulation Protein Gene Symbol: TSG Accession #: Q9GZX9 Gene ID: 57045 Source: E. Coli Appearance: Lyophilized powder Physical Form Description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Acetate, pH 6.0. Molecular Weight: 22.2 kDa Purity by SDS-PAGE: ≥95% Purity by HPLC: N/A Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg). Biological Activity: Determined by its ability to neutralize BMP-6 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is ≤ 2.0 μg/ml of TSG. Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. Storage Temp.: -20°C Shipping: Gel pack Background Information: Twisted Gastrulation Protein (TSG) is a secreted BMP binding protein structurally related to the BMP antagonists Chordin and Noggin. TSG can inhibit BMP activity by binding directly to BMP proteins, and can act either as a BMP4 agonist or antagonist (depending on the specific biochemical environment) by binding to the BMP4/Chordin complex. Recombinant human TSG is a 198 amino acid 22.2 kDa protein containing the BMP/TGFβ binding portion of the full length TSG protein. Amino Acid Sequence: CNKALCASDV SKCLIQELCQ CRPGEGNCSC CKECMLCLGA LWDECCDCVG MCNPRNYSDT PPTSKSTVEE LHEPIPSLFR ALTEGDTQLN WNIVSFPVAE ELSHHENLVS FLETVNQPHH QNVSVPSNNV HAPYSSDKEH MCTVVYFDDC MSIHQCKISC ESMGASKYRW FHNACCECIG PECIDYGSKT VKCMNCMF Handling: Centrifuge the vial prior to opening USAGE: For Research Use Only! Not For Use in Humans.
