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Glutathione Peroxidase 4 Antibody (Clone 1H11)
Monoclonal antibody to detect Glutathione Peroxidase 4 in human, mouse and rat samples.
Product Overview
Product Name: Glutathione Peroxidase 4 Antibody (Clone 1H11)
Antibody Target: Glutathione Peroxidase 4
Alternative Name: Phospholipid hydroperoxide glutathione peroxidase, mitochondrial, GPX4
Host: Mouse
Antibody Type: Monoclonal
Isotype: Mouse IgG1
Immunogen: Recombinant human protein purified from E.coli
Accession#: P36969
Gene ID: 2879
Appearance: Colorless liquid
Concentration: 1 mg/ml
Formulation: 100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol
Purification: Ammonium sulphate precipitation
Species Reactivity: Human, mouse, rat
Application: IP, ELISA.
Positive Control: WB and IP: HL60 cell lysate
Application & Usage: IP: 1-2 µl, ELISA.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic /mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |