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Glutaredoxin 1 Antibody (Clone 28C3)
Monoclonal antibody to detect Glutaredoxin 1 in human samples.
Product Overview
Product Name: Glutaredoxin 1 Antibody (Clone 28C3)
Antibody Target: Glutaredoxin 1
Alternative Name: Thioltransferase-1, GLRX, GRX
Host: Mouse
Antibody Type: Monoclonal
Isotype: Mouse IgG1
Immunogen: Recombinant human protein purified from E.coli
Accession#: P35754
Gene ID: 2745
Appearance: Colorless liquid
Concentration: 1 mg/ml
Formulation: 100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol
Purification: Ammonium sulphate precipitation
Species Reactivity: Human
Application: IP, ELISA.
Positive Control: WB and IP: Jurkat cell lysate
Application & Usage: IP: 1-2 µl, ELISA.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Glutaredoxin (Grx), also known as thiol transferase, is a small heat-stable oxidoreductase. Grxs form part of the glutaredoxin system, comprising NADPH, GSH and glutathione reductase, which transfers electrons from NADPH to glutaredoxins via GSH. First recovered in E.coli as GSH-dependent hydrogen donors for ribonucleotide reductase, Grx catalyzes GSH-disulfide oxido-reductase via two redox-active cysteine residues. The active sequence (Cys-Pro-Tyr-Cys) is conserved in a variety of species. The 12 kDa dithiol protein has a role in reduction of mixed disulfides in cells exposed to oxidative stress. Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |