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EGLN1 Antibody
Polyclonal antibody to detect EGLN1 in human, mouse and rat samples.
Product Overview
Product Name: EGLN1 Antibody
Antibody Target: EGLN1
Alternative Name: EGLN1, DKFZp 61F179, ECYT3, HIFPH2, HPH2, PHD2, SM20, SM20, ZMYND6.
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Recombinant protein of human EGLN1
Accession#: Q9GZT9
Gene ID: 54583
Appearance: Colorless liquid
Concentration: 1 mg/ml
Formulation: 100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Purification: Affinity purified
Species Reactivity: Human, mouse, rat
Application: Western blot, Immunohistochemistry
Positive Control: Western Blot: Various cell lysates
Application & Usage: Western blot: 1:500 – 1:2000, IHC: 1:50 – 1:200.
Storage Temp.: -20 °C
Shipping: Gel Pack
Background Descriptions: PHD1 (Egln2), PHD2 (Egln1), and PHD3 (Egln3) are members of the Egln family of proline hydroxylases. They function as oxygen sensors that catalyze the hydroxylation of HIF on prolines 564 and 402, initiating the first step of HIF degradation through the VHL/ubiquitin pathway. PHD1 is highly expressed in a wide array of tissues whereas PHD2 and PHD3 are expressed mainly in heart and skeletal muscle. The mRNA levels of PHD are upregulated by HIF through the hypoxia response element under low oxygen conditions. These three enzymes also exhibit different peptide specificity target proteins, PHD1 and PHD2 can hydroxylate both proline 402 and proline 564, but PHD3 can only hydroxylate proline 564. In addition to HIF, PHD enzymes have also has been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |