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BMI 1 Antibody
Polyclonal antibody to detect BMI 1 in human, mouse and rat samples.
Product Overview
Product Name: BMI 1 Antibody
Antibody Target: BMI 1
Alternative Name: RNF51, PCGF4
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Recombinant protein of human BMI1
Accession#: P35226
Gene ID: 648
Appearance: Colorless liquid
Concentration: 1 mg/ml
Formulation: 100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Purification: Affinity purified
Species Reactivity: Human, mouse, rat
Application: Western blot, Immunohistochemistry
Positive Control: Western Blot: K562 cell lysates
Application & Usage: Western blot: 1:500 – 1:2000, IHC: 1:50 – 1:200.
Storage Temp.: -20 °C
Shipping: Gel Pack
Background Descriptions: The polycomb group (PcG) of proteins contributes to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell cycle arrest. PcG proteins exist in two complexes that cooperate to maintain long term gene silencing through epigenetic chromatin modifications. The first complex, EEDEZH2, is recruited to genes by DNAbinding transcription factors and methylates histone H3 on Lys27. This histone methyltransferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex. Histone H3 methylation at Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119. Bmi1 is a component of the PRC1 complex, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16 INK4A and p19 ARF genes and is required for maintenance of adult hematopoietic and neural stem cells.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |