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UHRF1 Polyclonal Antibody
Polyclonal antibody to detect UHRF1 in human samples.
Product Overview
Product Name: UHRF1 Polyclonal Antibody
Antibody Target: UHRF1
Alternative Name: FLJ21925, ICBP90, MGC138707, Np95, RNF106, hNP95
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Recombinant protein of human UHRF1
Accession#: Q96T88
Gene ID: 29128
Appearance: Colorless liquid
Concentration: 1 mg/ml
Formulation: 100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Purification: Affinity purified
Species Reactivity: Human
Application: Western blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescene, CHIP
Positive Control: Western Blot: Various cell lysates
Application & Usage: Western blot: 1:500 – 1:2000, IHC: 1:50 – 1:200, IF: 1:20 – 1:50, IP: 1:20 – 1:50, CHIP: 1:20 – 1:50. However, the optimal conditions should be determined individually.
Storage Temp.: -20 °C
Shipping: Gel Pack
Background Descriptions: UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin protein ligase. It may participate in methylation dependent transcriptional regulation. UHRF1 binds to inverted 5'CCAAT3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD (UHRF1) binding to unmodified H3R2.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |