|
USP25 Polyclonal Antibody
Polyclonal antibody to detect USP25 (a DUB enzyme) in human, mouse and rat samples.
Product Overview
Product Name: USP25 Polyclonal Antibody
Antibody Target: USP25
Alternative Name: USP25, USP21
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Fusion protein of human USP25
Accession#: Q9UHP3
Gene ID: 29761
Appearance: Colorless liquid
Concentration: N/A
Formulation: 100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Purification: Affinity purified
Species Reactivity: Human, mouse, rat
Application: Western blot
Positive Control: Western Blot: BxPC-3 cell lysate
Application & Usage: Western blot: 1:500 – 1:1000 dilution. However, the optimal conditions should be determined individually.
Storage Temp.: -20 °C
Shipping: Gel Pack
Background Descriptions: Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action. There are five DUB subfamilies including the USP, UCH, OTU, MJD, and JAMM enzymes. USP25 (ubiquitin specific peptidase 25), also known as USP21, is a 1087 amino acid member of the peptidase C19 family. Expressed at high amounts in pancreas, kidney, skeletal muscle, testis, liver, heart, brain and lung, USP25 associates with the 26S proteasome and functions as a deubiquitinating enzyme in the Ub proteolytic pathway. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle specific isoform. The long isoform of USP25 is upregulated in myogenesis and is implicated in the pathogenesis of severe myopathies.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |