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DNA Methyltransferase 3a (Clone 64B1446) Antibody
Monoclonal antibody to detect DNMT3a in Human and Mouse samples
Product Overview
Product Name: DNA Methyltransferase 3a (Clone Clone 64B1446) Monoclonal Antibody
Antibody Target: DNA Methyltransferase 3a
Alternative Name: DNMT3, DNMT3A
Host: Mouse
Antibody Type: Monoclonal
Isotype: Mouse IgG1κ
Immunogen: Recombinant mouse Dnmt3a expressed in bacteria. The epitope was found to be located near the C-terminus (amino acids 705-908).
Accession#: O88508
Gene ID: 13435
Appearance: Colorless liquid
Concentration: N/A
Formulation: 50 μg of antibody in 100 μl PBS containing 0.05% BSA and 0.05% sodium azide.
Purification: Protein G purified
Species Reactivity: Human, mouse
Application: Western blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, Immunohistochemistry
Positive Control: Western blot: 293 cell lysate transfected with murine DNMT3a, 293 cell lysate transfected with murine DNMT3b
Application & Usage: Western blot: 2 μg/ml, ICC/IF: 5 μg/ml, ChIP/IHC (paraffin embedded sections). However, the optimal conditions should be determined individually.
Storage Temp.: -20 °C
Shipping: Gel Pack
Background Descriptions: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting, and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, three families of mammalian DNA methyltransferase genes have been identified which include DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The DNMT3 family members, DNMT3a and DNMT3b, are strongly expressed in embryonic stem (ES) cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of DNMT3b gene occur in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |