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Anti-BrdU antibody (Clone B1G5)
BrdU monoclonal Ab for studying cell proliferation, cytokinetics etc.
Product Overview
Product Name: Anti-BrdU antibody (Clone B1G5)
Antibody Target: BrdU
Alternative Name: BrdU, BUdR, BrdUrd
Host: Mouse
Antibody Type: Monoclonal
Isotype: Mouse IgG
Immunogen: BrdU covalently coupled to BSA
Accession#: N/A
Gene ID: N/A
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) in 10% glycerol in PBS.
Purification: Protein A purified
Species Reactivity: All species
Positive Control: N/A
Application: Immunohistochemistry, Immunoprecipitation, Immunocytochemistry, ELISA, Immunofluorescence, FACS
Application & Usage: The antibody can be used in IHC, IP, ICC, ELISA, IF, FACS. However, the optimal concentrations should be determined individually.
Storage Temp.: -80°C
Shipping: Gel Pack
Background Descriptions: Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analogue of thymidine. The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthesized DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. Antibody specific for BrdU is used to immunocytochemically detect the incorporated BrdU, which is a powerful tool to study cell proliferation, the cytokinetics of normal and neoplastic cells, etc.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |