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Human Recombinant SHP-1
A signaling molecule that regulates a variety of cellular processes
Product Overview
Product Name: Human Recombinant SHP-1
Alternate Name/Synonyms: Protein tyrosine phosphatase, non-receptor type 6 isoform 1, PTPN6, HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1L, SHP1.
Gene Symbol: PTPN6
Accession #: P29350
Gene ID: 5777
Source: E. coli
Appearance: Liquid
Physical Form Description: 1 mg/ml solution in 25 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 2 mM β-mercaptoethanol, 1 mM EDTA, 20% glycerol.
Molecular Weight: 34.3 kDa (300 aa)
Purity by SDS-PAGE: ≥95%
Purity by HPLC: N/A
Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method)
Biological Activity: >5,000 units/mg of SHP-1.
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: Gel Pack
Background Information: The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541) was cloned into an E. coli expression vector. The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). The recombinant SHP-1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid (Met) is attached at N-terminus.
Amino Acid Sequence: MGFWEEFESL QKQEVKNLHQ RLEGQRPENK GKNRYKNILP FDHSRVILQG RDSNIPGSDY INANYIKNQL LGPDENAKTY IASQGCLEAT VNDFWQMAWQ ENSRVIVMTT REVEKGRNKC VPYWPEVGMQ RAYGPYSVTN CGEHDTTEYK LRTLQVSPLD NGDLIREIWH YQYLSWPDHG VPSEPGGVLS FLDQINQRQE SLPHAGPIIV HCSAGIGRTG TIIVIDMLME NISTKGLDCD IDIQKTIQMV RAQRSGMVQT EAQYKFIYVA IAQFIETTKK KLEVLQSQKG QESEYGNITY
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |