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Anti-Autotaxin
Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Ca, B WB, IH(P) Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Autotaxin Antibody
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Specificity:
This antibody recognizes Autotaxin at the substrate binding region.
Molecular Weight:
~100/125 kDa observed.
3 isoforms are produced by alternative splicing with molecular weights of 99, 105, and 101 kDa. The calculated molecular weight of this protein is 99 kDa, but can be observed at ~125 kDa due to high glycosylation. An uncharacterized band may appear at ~35 kDa in some lysates.
Epitope:
Substrate binding region
Immunogen:
KLH-conjugated linear peptide corresponding to human Autotaxin at the substrate binding region.
Background Information:
Autotaxin (also known as Enpp2), an exo-enzyme that is upregulated in various cancers such as breast and lung cancer, is a secreted protein integral to the production of the lipid signaling molecule, lysophosphatidic acid (LPA). Wutotaxin is synthesized as a pre-pro-enzyme and stimulates cell proliferation, cell motility, and angiogenesis. Studies have shown increased expression of autotaxin in tumors is associated with increased tumor aggressiveness.
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Species Reactivity:
Human
Mouse
Rat
Canine
Bovine
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Species Reactivity Note:
Demonstrated to react with Human.
Predicted to react with Mouse, Rat, Canine, and Bovine based on 100% sequence homology.
Application Notes:
Western Blot Analysis: 0.5 µg/mL from a representative lot detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed with Tween.
Immunohistochemistry Analysis: A 1:400 dilution from a representative lot detected Autotaxin in human cerebral cortex and ductal carcinoma tissues.
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Control:
Oligodendrocyte precursor cell lysate
Quality Assurance:
Evaluated by Western Blot in oligodendrocyte precursor cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed without Tween.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q13822
Entrez Gene Number:
NP_001035181
Gene Symbol:
ENPP2
ATX
PDNP2
Alternate Names:
Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
E-NPP 2
Autotaxin
Extracellular lysophospholipase D
LysoPLD
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of |