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Anti-HepaCAM-1 Antibody
Species Reactivity Key Applications Host Format Antibody Type
H WB, IH(P) Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-HepaCAM-1 Antibody
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Specificity:
This antibody recognizes the cytoplasmic domain of HepaCAM-1.
Molecular Weight:
~53 kDa observed. Two isoforms at ~40 kDa and ~46 kDa are known to exist. This protein is known to be glycosylated in HT-1080 and HEPG2 cell lysates (Chung, M. M., et al. (2005). J Hepatol. 42(6):833-841.).
Epitope:
Cytoplasmic domain
Immunogen:
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human HepaCAM-1.
Background Information:
Gene HepaCAM, which is frequently silenced in hepatocellular carcinoma (HCC), encodes an Ig-like transmembrane glycoprotein. This glycoprotein serves as a type of cell adhesion molecule (CAM), which interacts with other CAMs on the adjacent cell surface or with proteins found in the extracellular matrix. It is observed to regulate cellular growth and cell-matrix adhesion. It also plays a role in cellular migration.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human. Predicted to react with Horse based on 100% sequence homology. Other homologies: Mouse, Rat, and Bovine (96% sequence homology).
Application Notes:
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected HepaCAM-1 in human liver and human breast carcinoma tissues.
Control:
Human liver tissue lysate
Quality Assurance:
Evaluated by Western Blot in human liver tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected HepaCAM-1 in 10 µg of human liver tissue lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q14CZ8
Entrez Gene Number:
NP_689935
Gene Symbol:
HEPACAM
Alternate Names:
Hepatocyte cell adhesion molecule
Protein hepaCAM
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry (Paraffin)
Product Name:
Anti-HepaCAM-1 Antibody
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
Detect the HepaCAM-1 protein using this Anti-HepaCAM-1 validated for use in WB & IHC. |