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Anti-phospho-RalA (Ser194)
Description:
Anti-phospho-RalA Antibody (Ser194)
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Specificity:
This antibody recognizes RalA phosphorylated at Ser194.
Molecular Weight:
~25 kDa observed. Uncharacterized bands may be observed at ~20 kDa, 100 kDa, and 130-170 kDa in some cell lysates.
Immunogen:
KLH-conjugated linear peptide corresponding to human RalA phosphorylated at Ser194.
Background Information:
RalA and RalB are Ras-family small monomeric GTPases that can be activated through a Ras-dependent mechanism. The two are highly homologous (89%). Ral proteins are proximal effectors of oncogenic Ras and their activity is needed to support tumorigenesis in certain cancers. Mutants of Ras that are defective in activating all known Ras effectors except Ral GEF are still able to induce invasive phenotypes in transfected cells, suggesting a role for Ral in these processes. RalA is required for anchorage-independent proliferation and tumorigeneisis by driving exocyst assembly and polarized exocytosis in epithelial cells.
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Species Reactivity:
Human
Bovine
Rat
Xenopus
Zebrafish
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Species Reactivity Note:
Demonstrated to react with Human. Predicted to react with Bovine, Rat, Xenopus, and Zebrafish based on 100% sequence homology.
Application Notes:
Western Blot Analysis: A representative lot detected phospho-RalA (Ser194) in certain primary and metastatic cell lysates, and in certain unmatched tumor tissue lysate samples.
Control:
PANC10.05 cell lysate
Quality Assurance:
Evaluated by Western Blot in PANC10.05 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected phospho-RalA in PANC10.05 cell lysate. Peptide inhibition was observed with the addition of phospho peptides but not with the addition of non-phospho peptides.
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Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P11233
Entrez Gene Number:
NP_005393
Gene Symbol:
RALA
Ral
Alternate Names:
Ras-related protein Ral-A
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Peptide Inhibition Assay
Product Name:
Anti-phospho-RalA (Ser194)
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit |