ODN M362 FITC

ODN M362 FITC
FITC labeled CpG oligonucleotide - Human / Mouse TLR9 ligand
CpG  ODNs are synthetic oligonucleotides that contain unmethylated CpG  dinucleotides in particular sequence contexts (CpG motifs). These CpG  motifs are present at a 20-fold greater frequency in bacterial DNA  compared to mammalian DNA. They have been shown to induce a coordinated  set of immune responses based on the activation of immune cells  primarily involved in the recognition of these molecules.
ODN M362 FITC is a C-Class CpG ODN specific for human / mouse TLR9. ODN M362 FITC can  be used to evaluate CpG ODN cellular uptake and localization by  confocal laser-scanning microscopy (excitation 495 nm, emission 520 nm)  or flow cytometry.
Specificity: human / murine TLR9 agonist
Working concentration: 10 ng -10 μg/ml
ODN M362 sequence
5’-tcgtcgtcgttc:gaacgacgttgat-3’ (25 mer)
Note: Bases are phosphorothioate (nuclease resistant), palindrome is underlined.
Spectral Properties of FITC
Excitation λ max: 495 nm
Emission λ max: 520 nm