ODN M362 Class C CpG oligonucleotide - Human / Murine TLR9 ligand CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs). These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. CpG ODNs are recognized by Toll-like receptor 9 (TLR9) leading to strong immunostimulatory effects. ODN M362 is a C-class CpG ODN specific for human / mouse TLR9. Specificity: human / murine TLR9 agonist Working concentration: 5 µM (10 μg/ml) Solubility: 5 mg/ml in water ODN M362 sequence 5’-tcgtcgtcgttc:gaacgacgttgat-3’ (25 mer) Note: Bases are phosphorothioate (nuclease resistant), palindrome is underlined. Quality control - TLR9 activity is tested using HEK-Blue™ TLR9 cells. - The absence of bacterial contamination (endotoxins, peptidoglycans) is controlled using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.