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Blasticidin
Blasticidin is an efficient selective antibiotic that acts on both eukaryotic and prokaryotic cells. Blasticidin is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes that inhibits protein synthesis by interfering with the peptide-bond formation in the ribosomal machinery.
Resistance to blasticidin is conferred by the blasticidin resistance gene from Bacillus cereus (bsr), which codes for blasticidin-S deaminase.
Cell death induced by blasticidin is rapid and cell lines carrying a blasticidin resistance gene can be obtained in less than a week. Typically, mammalian cells are sensitive to blasticidin concentrations of 1-10 µg/ml, and bacteria to 25-100 µg/ml.
Working concentration:
- 25-100 µg/ml in bacteria
- 1-30 µg/ml in mammalian cells
Purity: controlled by HPLC >95%
Formula: C17H26N8O5, 1 HCl
Quality control:
Blasticidin hydrochloride is supplied as colorless solution in HEPES buffer (100% active compound), pH 7.5, filtered to sterility for customer convenience and cell culture tested, or as a powder.
Activity controlled by bioassays on bacteria and mammalian cell lines. |