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Anti-phospho KSR1 (Ser404), clone EP2358Y, Rabbit Monoclonal
Species Reactivity Key Applications Host Format Antibody Type
H WB, ICC Rabbit Diluted Culture Supernatant Monoclonal Antibody
Description:
Anti-phospho KSR1 (Ser404) Antibody, clone EP2358Y, rabbit monoclonal
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Specificity:
This antibody recognizes KSR1 phosphorylated at Ser404.
Molecular Weight:
~110 observed
Epitope:
Phosphorylated Ser404
Immunogen:
Synthetic phospho-peptide corresponding to human KSR1 phosphorylated Ser404.
Modifications:
Phosphorylation
Clone:
EP2358Y
Isotype:
IgG
Background Information:
Kinase suppressor of Ras (KSR) is a conserved component of the Ras pathway that interacts directly with MEK and MAPK. It has been shown that KSR1 translocates from the cytoplasm to the cell surface in response to growth factor treatment and that this process is regulated by MARK3 (1). MARK3 phosphorylates serine 309 and serine 404 on KSR1. While, PPP2CA dephosphorylates serine 404 on KSR1. MARK3 seems to be a positive regulator of the beta-catenin pathway and an inhibitor of the JNK pathway. These findings show that MARK3, a regulator of polarity, is also a modulator of Wnt-beta-catenin signaling, indicating a link between two important developmental pathways (2).
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with human.
Application Notes:
Immunocytochemistry Analysis: 1:100-1:250 dilution from a representative lot detected KSR1 in Sh-SY-5Y cells.
Control:
Calyculin A untreated and treated HeLa cell lysates
Quality Assurance:
Evaluated by Western Blot in Calyculin A untreated and treated HeLa cell lysates.
Western Blot Analysis: 1:2,000 dilution of this antibody detected KSR1 in Calyculin A untreated and treated HeLa cell lysates.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
Q8IVT5
Entrez Gene Number:
Q8IVT5
Gene Symbol:
KSR
RSU2
OTTHUMP00000162931
Alternate Names:
kinase suppressor of ras 1
kinase suppressor of ras
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
UniProt Summary:
FUNTION: Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.
SUBUNIT STRUCTURE: Interacts with HSPCA/HSP90, YWHAB/14-3-3, CDC37, MAP2K/MEK, MARK3, PPP2R1A and PPP2CA. Also interac |