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Anti-pan-SAPAP, clone N127/31
Species Reactivity Key Applications Host Format Antibody Type
R IHC, WB Mouse Purified Monoclonal Antibody
Description:
Anti-pan-SAPAP Antibody, clone N127/31
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Molecular Weight:
Isoforms between ~90-120 kDa observed. Other isoforms may be observed in some lysates.
Immunogen:
Recombinant protein corresponding to rat SAPAP.
Clone:
N127/31
Isotype:
IgG2bκ
Background Information:
SAPAP1 is a member of the SAPAP family of proteins and is thought to be instrumental in the postsynaptic scaffold of neurons. SAPAP1 may function to assemble various other proteins at synapses. These functional proteins include scaffolding proteins, signaling proteins and cytoskeletal proteins. SAPAP1 is primarily found in the brain and more specifically in the cell body zones.
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Species Reactivity:
Rat
Species Reactivity Note:
Demonstrated to react with rat.
Application Notes:
Immunohistochemistry Analysis: 1:400 dilution from a previous lot detected SAPAP in rat schwan cells (putamen area) and rat dopaminergic neurons.
Immunofluorescence Analysis: A previous lot of this antibody was used by an independent laboratory in cultured rat hippocampal pyramidal cells and interneurons. (Courtesy of Dieter Edbauer and Morgan Sheng, Picower Institute, MIT.)
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Control:
Rat brain membrane tissue lysate
Quality Assurance:
Evaluated by Western Blot in rat brain membrane tissue lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected SAPAP on 10 µg of rat brain membrane tissue lysate.
Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P97836
Entrez Gene Number:
NP_075235
Gene Symbol:
DLGAP1
GKAP
DAP-1-BETA
DAP-1
SAPAP1
DAP-1-ALPHA
hGKAP
DAP1
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Alternate Names:
discs, large (Drosophila) homolog-associated protein 1
SAP90/PSD-95-associated protein 1
disks large-associated protein 1
Guanylate kinase-associated protein
PSD-95/SAP90 binding protein 1
PSD-95/SAP90-binding protein 1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunohistochemistry
Western Blotting
Entrez Gene Summary:
binds the guanylate kinase-like domain of the PSD-95 family of synaptic ion channel clustering proteins; may play a role in protein-protein interactions at the synaptic junction [RGD].
UniProt Summary:
FUNCTION: Part of the postsynaptic scaffold in neuronal cells.
SUBUNIT STRUCTURE: Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein. Cell junction › synapse › postsynaptic cell membra |