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Anti-ADAM 22, clone N46/30
Species Reactivity Key Applications Host Format Antibody Type
R, M IHC, IP, IF Mouse Purified Monoclonal Antibody
Description:
Anti-ADAM 22 Antibody, clone N46/30
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Specificity:
This antibody recognizes ADAM 22 at the cytoplasmic domain.
Molecular Weight:
Calculated Mw is ~100 kDa
Epitope:
Cytoplasmic domain
Immunogen:
Recombinant protein corresponding to mouse ADAM 22 at the cytoplasmic domain.
Clone:
N46/30
Isotype:
IgG2bκ
Background Information:
ADAM 22 belongs to the ADAM gene family which have been shown to bind integrin and therefore may have a part in cell to cell or cell to matrix interactions. ADAM 22 is unique in the fact that it is only observed in the nervous system and predominantly in the brain. ADAM 22 is attached by cytoskeletal scaffolds to the postsynaptic density and is a receptor for LGI1.
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Species Reactivity:
Rat
Mouse
Species Reactivity Note:
Demonstrated to react with rat. Predicted to react with mouse based on immunogen design.
Application Notes:
Immunohistochemistry Analysis: 1:400 dilution of this antibody detected ADAM 22 in rat neurons/myelin in putamen area.
Western Blot Analysis: A previous lot of this antibody was used by an independent laboratory to detect ADAM 22 in rat and mouse brain tissue lysate (James Trimmer, UC Davis/NIH NeuroMab Facility).
Immunoprecipitation Analysis: A previous lot of this antibody was used by an independent laboratory in IP. (Ogawa, Y., et al. (2010). J Neurosci. 30(3):1038–1048.)
Immunofluorescence Analysis: A previous lot of this antibody was used by an independent laboratory in IF. (Ogawa, Y., et al. (2010). J Neurosci. 30(3):1038–1048.)
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Control:
Rat myelinated neurite tissue
Quality Assurance:
Evaluated by Immunohistochemistry in rat myelinated neurite tissue.
Immunohistochemistry Analysis: 1:400 dilution of this antibody detected ADAM 22 in rat myelinated neurite tissue.
Purification Method:
Protein G Purified
Presentation:
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q9R1V6
Entrez Gene Number:
NP_001007221
Gene Symbol:
MDC2
ADAM 22
ADAM22
Alternate Names:
ADAM metallopeptidase domain 22
Metalloproteinase-disintegrin ADAM22-3
a disintegrin and metalloproteinase domain 22
disintegrin and metalloproteinase domain-containing protein 22
Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 2
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunohistochemistry
Immunoprecipitation
Immunofluorescence
UniProt Summary:
FUNCTION: Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation (By similarity). Neuronal receptor for LGI1.
SUBUNIT STRUCTURE: Interacts with LGI1. Can bind to LGI4. |