BACE-1, HIS-TAG, HUMAN, RECOMBINANT

BACE1, His•Tag®, Human, Recombinant, NSO Cells
Recombinant, human BACE-1 (amino acids 1-460) fused at the C-terminus to a His•Tag® sequence and expressed in NSO cells. Supplied as a mixture of the ~68-70 kDa proenzyme and the ~65-67 kDa mature enzyme. BACE-1 is an aspartic protease that has been shown to cleave the amyloid precursor protein β site in the brain to generate the amyloid β peptide. MW: ~65-70 kDa under reducing conditions.
产品信息
Form  Lyophilized
Formulation  Lyophilized from sterile-filtered PBS.
Preservative  None
Specific activity  ≥1 pmol/min/µg protein
Activity  ≥1 pmol/min/µg as measured by the cleavage of 10 µM MCA-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(DNP)-Arg-Arg-NH2 fluorescent substrate using 100 ng BACE-1 in 0.1 M sodium acetate (pH 4.0) at room temperature.
Purity  ≥90% by SDS-PAGE
Contaminants  Endotoxin: ≤1 EU/µg BACE-1
Solubility  H2O
商店和运输信息
Storage  -20°C
Ship  Blue Ice Only
Standard Handling