ADAM10, HIS-TAG, MOUSE, RECOMBINANT

ADAM10, His•Tag®, Mouse, Recombinant, S. frugiperda
Kuzbanian Metalloproteinase
Recombinant, mouse ADAM10 (mature, active mouse ADAM10 ectodomain; amino acids 19-673) fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. An integral membrane proteinase involved in notch signaling. Also considered as an α-secretase candidate.
产品信息
Form  Lyophilized
Formulation  Lyophilized from a filtered solution of 100 mM NaCl, 25 mM MES, 10 µM ZnCl2, 0.02% BRIJ®-35 Detergent, pH 5.5.
Molar mass  60000
Applications 
Immunoblotting (Western Blotting)
Substrate Cleavage Assay
Avoid freeze/thaw  是的
Specific activity  ≥5 pmol/min/µg
Activity  ≥5 pmol/min/µg as measured by the cleavage of 10 µM of the fluorescent substrate MCA-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH2 (Cat. No. 616402) by 100 ng of the enzyme in 100 µl of reaction buffer at 37°C.
Purity  ≥90% by SDS-PAGE
Contaminants  Endotoxin: ≤1 EU/µg ADAM10
Solubility  25 mM Tris-HCl buffer, pH 7.5
商店和运输信息
Storage  -20°C
Ship  Dry Ice Only
Standard Handling