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pIEx™-1 DNA
InsectDirect™ System- pIEx™-1 DNA
The pIEx™ vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. pIEx-1 contains N-terminal His•Tag and S•Tag coding sequences, and a C-terminal HSV•Tag coding sequence. N-terminal vector encoded sequences can be completely removed by cloning into the PshA I site and cleaving the fusion protein with enterokinase.
pIEx-1 is also available as an Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.
For sequencing or PCR of recombinants in pIEx-1, refer to the table below to choose the appropriate primers.
Vector 5′ Primer 3′ Primer alt 5′ Primer
pIEx-1 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
pIEx-2 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
pIEx-3 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
pIEx-4 IE1 Promoter Primer AS S•Tag 18mer Primer
NA
pIEx-5 IE1 Promoter Primer AS S•Tag 18mer Primer
NA
pBiEx-1 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
T7 Promoter Primer
pBiEx-2 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
T7 Promoter Primer
pBiEx-3 IE1 Promoter Primer AS S•Tag 18mer Primer T7 Promoter Primer
商店和运输信息
Storage -20°C
Ship Shipped with Blue Ice or with Dry Ice
Standard Handling |