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pIEx™-2 Ek/LIC Vector Kit
InsectDirect™ pIEx™-2 Ek/LIC Vector Kit
The pIEx™-2 Ek/LIC Vector is designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. This vector features the hr5 enhancer and the IE1 (immediate early) promoter to direct expression in insect cells. This promoter/enhancer combination recruits endogenous insect cell transcription machinery, thereby avoiding baculovirus infection and the associated cytotoxic effects. Cloning sites are available for producing fusion proteins containing a GST•Tag™ sequence for potential enhanced solubility, detection, purification and quantification as well as His•Tag® and S•Tag™ sequences for detection, purification and quantification. All N-terminal tags are cleavable. A C-terminal HSV•Tag® sequence serves as a cloning option to permit detection of target protein with the HSV•Tag Monoclonal Antibody.
相关产品
71237 pIEx™-1 Ek/LIC Vector Kit
71245 pIEx™-3 Ek/LIC Vector Kit
产品信息
Components
• 1 µg EK/LIC Vector
• 8 µl Ek/LIC b-Gal Control Insert
• 25 U LIC-qualified T4 DNA Polymerase
• 50 µl T4 DNA Polymerase Buffer
• 100 µl 100 mM DTT
• 40 µl 25 mM dATP
• 50 µl 25 mM EDTA
• 1.5 ml Nuclease-free Water
• 22 × 50 µl NovaBlue GigaSingles™ Competent Cells
• 5 × 2 ml SOC Medium
• 10 µl Test Plasmid
Declaration pIEx is a trademark of EMD Biosciences, Inc., Madison, WI USA
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS |