N-GLYCOSIDASE F, C. MENINGOSEPTICUM

N-Glycosidase F, Elizabethkingia meningosepticum
Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1,3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.
产品信息
Form  Liquid
Formulation  In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.
Molar mass  36000
Do not freeze  是的
Specific activity  ≥20,000 units/mg protein
Unit definition  One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 nmol denatured ribonuclease B per min at 37°C, pH 7.5. Cleavage is monitored by SDS-PAGE. One unit of N-Glycosidase F is equal to 1 IUB milliunit.
Activity  ≥4500 units/ml
Contaminants  Proteases: none detected.
EC number  3.5.1.52
CAS number  83534-39-8
商店和运输信息
Storage  +2°C to +8°C
Ship  Blue Ice Only
Standard Handling