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Complement C3, Human
Native, human C3 complement component. Glycoprotein composed of two non-identical disulfide-bonded subunits with M.W. of 115 kDa (α) and 75 kDa (β). Present in normal human serum at 1.25 mg/ml. Classical and alternative activation pathways of complement converge at C3 step. Activation via either pathway can result in assembly of C3-cleaving enzymes (C3 convertases) on target surfaces. Both C3 convertases cleave the C3 α-chain at peptide bond 77 resulting in production of C3a (M.W. 9083) and C3b fragments (M.W. 180,000). Released C3a peptide is one of the three complement anaphylatoxins. The nascent C3b fragment can form a covalent ester bond with target surface. This covalent attachment of C3b to target acceptors is required for continuation of complement activation.
产品信息
Form Liquid
Formulation In PBS, pH 7.2.
Molar mass 190000
Avoid freeze/thaw 是的
Biological activity >3,000 C3H50 units/mg; ≥60% of the C3 activity in normal human serum on a mg/mg basis.
Purity ≥95% by SDS-PAGE
Contaminants IgG, IgA, IgM, C4, functionally active C5, albumin or ceruloplasmin: ≤trace amounts
Source Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
CAS number 80295-41-6
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
Standard Handling |