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Beta-Gamma Catenin [pY654/pY643] Mouse mAb (clone 1 B11)
The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. Additionally, β-catenin is a substrate of both receptor and non-receptor tyrosine kinases. Tyrosine 86 and tyrosine 654 are substrates of EGFR and Src family kinases while tyrosine 142 is a substrate of FER tyrosine kinases.
Isotype: Mouse IgG1
Clone/PAD: 1B11
Immunogen: Phosphopeptide containing amino acid sequence VAT[pY]AAA, conjugated to hemocyanin.
Specificity: This antibody specifically recognizes β-catenin when phosphorylated at tyrosine 654 (90 kDa) and Ü-catenin when phosphorylated at tyrosine 643 (70 kDa). Due to sequence homologies of the phospho-epitope surrounding tyrosine 654, there may be cross-reactivity with other proteins (e.g., Annexin A11 (56 kDa), Desmoplakin (81 kDa), TNFÉ-induced protein 2 (73 kDa), ribosomal protein S6 kinase alpha 6 (90 kDa)).
Reactivity: Cn, Hu, Ms
Applications: WB
Reactivity: Human,
Mouse
Validated Application: Western Blot
Host: Mouse
Clonality: Monoclonal
Clone ID: 1 B11
Target Gene: Catenin beta-Gamma [pY654/pY643]
Label or Dye: Unconjugated
Target Modification State: Phospho
Product Size: 50 µL
Shipping Condition: Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Store in freezer (-5 to -30°C). |